摘要
为完善西瓜离体再生体系,以23种基因型材料的西瓜无菌苗子叶为试材,运用方差分析筛选高效基因型材料及芽诱导最适培养基。结果表明:基因型不同的材料,诱导效果有明显差异。筛选的再生高效基因型为鲁圆、三白、FW351、PM2和京母。鲁圆诱导效果最好,最高诱导率达到90.0%,与18种基因型材料的诱导差异达到了显著水平;三白、FW351、PM2和京母的诱导效果次之,诱导率分别为85.0%、82.5%、82.5%和80.0%。筛选的芽诱导最适培养基为MS+6-BA3.0mg/L。
Twenty three genotypes of Citrullus lanatus were compared in vitro for their efficiency of plantlet regeneration from cotyledons and the suitable medium. The data were analyzed using ANOVA. The results showed that the regeneration frequencies were different among genotypes with Luyuan ,PM2 ,FW351 ,Sanbai and Jingmu being the more regenerative genotypes. The highest regeneration rate of Luyuan was 90.0%, significantly higher than other 18 genotypes. The regeneration rate of PM2, FW351 ,Sanbai and Jingmu were 85.0%, 82.5%, 82.5% and 80.0%, respectively. MS＋6-BA 3.0 mg/L was the suitable culture medium for shoot organogenesis.
出处
《中国瓜菜》
2008年第3期5-9,共5页
China Cucurbits And Vegetables
基金
北京市科委资助项目(Z07070500430701,Z07080501570702)
关键词
西瓜
组织培养
基因型材料
筛选
方差分析
Citrullus lanatus
Tissue culture
Genotype
Scereen
Variance analysis
