摘要
利用Kan处理甜瓜种子、幼苗,选择不同品种、不同时期Kan临界浓度,对花粉管通道法转化的甜瓜种子进行早期筛选。结果表明,鲁薄1号甜瓜(薄皮)对Kan反应较乌克兰、雪里华甜瓜(厚皮)敏感,种子较幼苗敏感,子叶承受的Kan压力是种子的3-4倍。子叶涂抹法是较适宜的筛选方法。PCR及Southern杂交检测证明,Kan对大量转化甜瓜种子进行早期筛选是可行的。
Different concentrations of Kanamycin （Kan）for treating seeds and seedlings of different melons were studied for early screening transgenic melon achieved through pollen-tube mediated transformation. The results indicated that Lubo No. 1 （thin-skinned melon）reacted to Kan more sensitively than Wukelan and Xuelihua （thick-skinned）,seeds was more sensitive than seedlings. Cotyledon resistance to Kan was 3-4 times of seeds. Method of Kan smearing cotyledons with Kan was suitable. Detection of PCR and Southern showed that screening transgenic seeds by Kan treatment is feasible.
出处
《中国瓜菜》
CAS
2008年第3期9-12,共4页
China Cucurbits And Vegetables
基金
山东省农业良种工程项目--蔬菜种质资源创新[鲁科农字(2004)113号]
