番茄花药培养的影响因素研究

Studies of Influncing Factors on Anther Culture of Tomato(Lycopersicon esculentum Mill.)

以4个番茄F1代杂交种的花药为试材,通过正交试验设计,研究不同材料基因型、不同低温处理时间、不同浓度的2,4-D、蔗糖和硝酸银对番茄花药培养中组织褐化和愈伤组织生成的影响。结果表明:硝酸银和材料基因型是组织褐化的主要影响因素,其次是2,4-D,而蔗糖和低温处理的影响较弱。06-176经低温48h处理,培养基中添加2,4-D0.1mg/L、蔗糖120g/L和硝酸银50μmol/L是减轻组织褐化的最佳培养组合;而2,4-D是愈伤组织生成最主要的影响因素,其次是材料基因型和低温处理,蔗糖和硝酸银的影响较弱。06-506经24h低温处理,培养基中添加2,4-D1.0mg/L、蔗糖30g/L和硝酸银50μmol/L是提高愈伤组织发生率的最佳培养基组合。

Four F, hybrids of tomato were used in the experiment with MS as the basal medium to study the effects of genotypes, low temperature, 2,4--D, sucrose and AgNO3 on the anther culture of tomato through orthogonal tests. The results showed that AgNO3 and genotypes was the most effective element for browning of the tissues, followed by 2,4--D with the least effective element for sucrose and low temperature. The optimized combination of reduce browning of the tissues was derived with low temperature 48 h, 0. 1 mg/L 2,4--D, 120 g/L sucrose and 50μmol/L Ag- NO3 on 06--176; 2,4--D have a higher effect on callus yields, followed by genotypes and low temperature with the least effective element for sucrose and AgNO3. The optimized combination of improve callus yields was derived with low temperature 24 h,1. 0 mg/L 2,4--D, 30 g/L sucrose and 50μmol/L AgNO3 on 06--506.