奥沙利铂与羟基喜树碱单用及联合应用对人大肠癌疗效的研究

A study on efficacy of hydroxycamptothecin and oxaliplatin used singly and in combination to colorectal carcinoma

目的观察奥沙利铂(L-OHP)、羟基喜树碱(HCPT),顺铂(CDDP),5-氟尿嘧啶(5-Fu),丝裂霉素(MMC)及表阿霉素(EPI)在体外单用及两药联用时对人大肠癌标本的敏感性。方法MTT法检测药敏结果,按中效原理计算不同药物联用的效果。结果大肠癌敏感率由高至低的单药依次为L-OHP>HCPT>CDDP>5-FU>MMC>EPI。联合用药组依次为L-OHP+HCPT、L-OHP+5-Fu、HCPT+MMC、HCPT+CDDP、HCPT+5-Fu与MMC+5-Fu。L-OHP与HCPT对大肠癌敏感性均好。尤其是低分化癌。EPI对大肠癌细胞不敏感。L-OHP+HCPT联合用药都对大肠癌最敏感。结论L—OHP与HCPT单用及联合用药对大肠癌细胞最敏感,尤其对低分化的癌的效果优于CDDP、MMC与5-Fu单药及联用。

Objective To evaluate the sensitivity in vitro of cultured cancer cells of operative resected colorectal carcinoma specimens from patients to Oxaliplatin （L-OHP）, Hydroxycamptothecin （HCPT）, Mitmycine （MMC）, Epimbin （EPI）, Cisplatin （CDDP） and 5-Fluorouracil （5-FU） when used singly and in combination of two. Methods MTr assay was performed for chemotherapeutic drugs sensitivity test, and the concentrations of each chemotherapeutic agent which is in different inhibitive rate were caculated according to the medlan-effect principles. Results The results of sensitivity test from high to low when used singly were as follows： L-OHP〉HCPT〉CDDP〉5-FU〉MMC〉 EPI, and in combination of two agents from high to low were as follows： L-OHP＋HCPT〉 L-OHP＋5-Fu〉HCPT＋ MMC〉HCPT＋CDDP〉 HCPT＋5-Fu 〉MMC＋5-Fu. It＇s sensitive to colorectal carcinoma when used in combination for L-OHP and HCPT, specially to low differentiation carcinomas. EPI is not sensitive to colorectal cancer. Conclusion It＇s more sensitive for colorectal carcinoma when used singly and combined with L-OHP and HCPT especially for low differentiation carcinomas.