柴芩承气汤对急性胰腺炎大鼠腺泡细胞外分泌功能的影响

Effects of ChaiQinChengQi Decoction on Exocrine Function of Pancreatic Acinar cells of Acute Pancreatitis Rats

目的研究柴芩承气汤(CQCQ-D)对急性胰腺炎(AP)大鼠胰腺腺泡细胞外分泌功能和腺泡细胞内钙离子浓度([Ca2+]i)变化的影响。方法SD大鼠灌喂CQCQ-D以制备柴芩承气汤含药血清(CQCQ-S);SD大鼠分为AP组和假手术组,分离胰腺腺泡细胞并与CQCQ-S共同孵育,观测腺泡细胞淀粉酶分泌水平和[Ca2+]i变化。结果AP大鼠腺泡细胞淀粉酶分泌水平较假手术组降低(P<0.01),CQCQ-S使AP大鼠腺泡细胞淀粉酶分泌水平进一步降低(P<0.05);[Ca2+]i随AP病程延长而升高(P<0.05),CQCQ-S可抑制AP大鼠腺泡细胞内[Ca2+]i升高(P<0.05)。结论CQCQ-D对AP大鼠的胰腺腺泡细胞的外分泌功能和腺泡细胞内钙超载有抑制作用。

Objective To investigate the effects of Chaiqinchengqi decoction（CQCQ-D）on exocrine function of pancreatic acinar cells and the changes of intracellular calcium concentration（[Ca^2＋]i）of acute pancreatitis（AP）rats,so as to explore the mechanism of CQCQ-D.Methods Acute pancreatitis model was induced in Sprague-Dawley rat by ligation of bile-pancreatic duct,pancreatic acinar cells were isolated by collagenase digestion and in vitro co-incubated with the drug serum containing Chaiqingchengqi decoction（CQCQ-S）which was collected from normal rats intragastrically fed on CQCQ-D,then the amylase activity secreted into the buffer was assayed and intracellular fluorescent intensity were observed and analyzed to investigate the changes of intracellular calcium concentration（[Ca^2＋]i）.Results Amylase secretion in AP group was lower than that in sham-operation（SO）group（P〈0.01）,while CQCQ-S treatment led to more significant decrease（P〈0.05）;[Ca^2＋]i elevated in AP rats（P〈0.05）,while the level of [Ca^2＋]i elevation was redu ced after CQCQ-S treatment（P〈0.05）.Conclusion CQCQ-D has inhibitive effects on exocrine function of pancreatic acinar cells and the calcium overload of AP rats.