激光诱导恒河猴实验性脉络膜新生血管的研究

Experimental Choroidal Neovascularization Induced by Laser in the Eyes of Rhesus Monkeys

目的通过激光诱导恒河猴眼底脉络膜血管新生,建立与人类脉络膜新生血管类似的动物模型。方法8只成年恒河猴,采用532nm激光围绕黄斑中心凹光凝,光凝前及光凝后20d、34d、48d进行眼底彩色照相、荧光素眼底血管造影、光学相干断层扫描,光凝后49d处死动物进行组织学和免疫荧光化学染色检查。结果68.8%激光斑形成实验性脉络膜新生血管,荧光素眼底血管造影表现为早期强荧光斑,后期荧光素渗漏超越激光光斑边缘;光学相干断层扫描出现神经上皮下的高反射光团,局部视网膜增厚,病灶可维持长达48d,形成的脉络膜新生血管为含有与增殖相关的CD31、CD105染色阳性内皮细胞成分的纤维血管增殖膜并伴视网膜水肿,色素颗粒内移,外核层缺失。结论激光诱导的恒河猴脉络膜新生血管模型成模率高,模型稳定持久,是研究治疗与脉络膜新生血管相关眼底病理想的动物模型。

Objective To establish an experimental model of choroidal neovascularization（CNV）through perimacular laser treatment in the eyes of rhesus Monkey.Methods The experimental CNV was induced by perimacular laser injury in the eyes of 8 rhesus monkeys and confirmed by a comparison before and after the laser treatment（20 d,34 d,48 d）with fluorescence fundus angiography（FFA）and optical coherence tomography（OCT）.Results Classic CNV similar to human CNV appeared in 68.8% of the laser spots.Hypofluorescence in the early phase and fluorescence leakage in the late phase were detected by the FFA.High reflect light echogenic mass and retina edema were detected by the OCT.The histopathologic examinations found proliferated fiber-vasculosa membranes in the laser burnt spots.The pathological changes lasted 48 days until the monkeys were killed.Conclusion The laser induced experimental CNV in rhesus monkey has a high prevalence and stability,which maintains a long period.It is an ideal experimental model for studying the pathologic mechanism of CNV and effective treatment for CNV.