牙釉基质蛋白诱导牙釉质的再矿化研究

The Study on the Enamel Remineralization by Enamel Matrix Proteins' Inducing

目的研究牙釉基质蛋白对牙釉质再矿化的影响。方法将利于与蛋白结合的羧基基团,通过紫外光辐射接枝在牙釉质缺损表面,加入到含SD大鼠牙釉基质蛋白的磷酸盐琼脂-醋酸钙溶液体系中,应用扫描电镜观察牙釉质表面经处理后的形态学改变,进而分析牙釉基质蛋白对羟基磷灰石晶体的组成和形貌的调控作用。结果在添加牙釉基质蛋白的凝胶体系中,牙釉质缺损表面可以见到羟基磷灰石生长晶体,晶体表现出了良好的结晶度,组成上为含有少量CO32-取代的羟基磷灰石晶体。结论在37℃恒温水浴,添加牙釉基质蛋白的凝胶体系中,牙釉基质蛋白对羟基磷灰石晶体的成核及生长有重要模板作用,证明牙釉基质蛋白能够诱导牙釉质再矿化。

Objective To find the enamel matrix proteins on the impact of enamel mineralization through experiments.Methods A combination of protein and beneficial carboxyl groups was grafted on the surface of enamel defects of rats through UV radiation then put into the enamel matrix proteins of calcium phosphate agar acetate solution systems,through scanning enamel surface with the electron microscopy to observe the morphological changes of enamel then analyse the regulation that enamel matrix proteins have done to the white hydroxyapatite crystals on the composition and morphology.Results In the enamel matrix protein added gel system,we can see the growth of hydroxyapatite crystals,and crystal showed a good degree of crystallinity and contained a small amount of CO3^2-substituted hydroxyapatite crystals.Conclusion The temperature at 37 ℃ water bath,after adding the enamel matrix proteins to gel system,the new hydroxyapatite crystals were numerous which proved that enamel matrix proteins played an important role innucleation and growth of hydroxyapatite crystal,so it could be indicated that enamel matrix proteins could induce the enamel remineralization.