转导素β-TrCP在小鼠毛囊发育中的表达与意义

Transducin β-TrCP Expressed in Developmental Hair Follicle of Mice

目的观察转导素β-TrCP在出生后乳鼠须毛囊的表达情况,并探讨β-TrCP在毛囊发生中的作用。方法分别取胚胎18d、出生后第1d、6d小鼠头部标本,40g/L多聚甲醛固定,石蜡包埋,用LsAB法观察β-TrCP在须毛囊中的表达分布情况,并进行图像分析。结果在出生后不同发育时期的须毛囊中,β-TrCP在毛囊发育时期的毛干细胞与毛小皮细胞胞浆阳性表达;鞘小皮细胞胞浆、内根鞘Huxley层、外根鞘、间充质细胞胞浆阳性表达。内根鞘亨勒层细胞胞浆与结缔组织鞘细胞胞浆阴性表达。图像分析显示出生第6d表达量与胚胎18d表达量的差异有统计学意义(P<0.01)。结论β-TrCP在小鼠毛囊发育中呈现特征性表达。提示β-TrCP对于毛囊发育的分子信号通路正常传递具有重要作用。

Objective To identify the expression of antagonist β-TrCP protein in Sonic hedgehog signal transduction pathway and Wnt signal transduction pathway in hair follicle tissues.Methods The heads of day 18 embryo,and one day and six days old postnatal mice were acquired and treated with 40 g/L paraformaldehyde fixation for 48 h and paraffin embedding.The expression of β-TrCP proteins was examined using LsAB（labelled streptavidin-biotin）method.Results β-TrCP proteins were expressed in the cytoplasm of the hair stems of hair follicle,hair cuticle,cuticle of root sheath,Huxley＇s layer of internal root sheath cells,external root sheath and mesenchymal tissues,but not in connective tissue sheath and Henle＇s layer of internal root sheath.Conclusion β-TrCP express in the developmental hair follicle tissues,which implicates that β-TrCP regulate the developmental hair follicle by mediating the signal transduction pathways.