幼建鲤维生素C缺乏的病理组织学观察

Histopathological observation of vitamin C deficiency in juvenile Cyprinus carpio var.Jian

将192尾体重为11.18g±0.57g(P>0.05)的健康幼建鲤随机分为2组,分别喂以含维生素C60mg/kg的半纯合饵料和维生素C缺乏(0mg/kg)饵料133d,观察幼建鲤器官组织的病理学变化。试验结果显示,维生素C缺乏组幼建鲤出现肝细胞变性、坏死,肾小管上皮细胞变性、坏死、脱落,脾网状基质细胞肿胀,心肌纤维肿胀、变性,肌纤维变性、坏死等病理变化;电镜观察显示,胰腺腺细胞中的酶质颗粒溶解呈空泡状,肌纤维细胞断裂,肠上皮细胞绒毛断裂,脾网状基质肿胀。表明幼建鲤维生素C缺乏可导致组织器官出现不同程度的病理损伤。

A total 192 healthy Cyprinus carpiovar. Jian carps（body weight 11.18 g±0.57 g,P〉0.05） were divided randomly into groups A and B. Group A was fed with the semi-purified diet supplemented with 60 mg vitamin C per kg ascontrol and Group B was fed with the experimental diet without vitamin C for 133 days. The histopathological observation was carried out. In Group A,hepatocytes were degenerated and necrotic,renal tubular epithelial cells were degenerated, necrotic and exfoliated, splenic reticular stromal cells were swelled,myocardial fiber were swelled and degenerated,and muscle fiber were degenerated and necrotic. Under electronic microscopy, hepatin and enzyme particles were dissolved and looked like cavitation in pancreas cell,muscular fibrocyte and intestinal epithelium villi were ruptured,and splenic reticular stromal cells were swelled. The results revealed that different histopathological injuries were caused by vitamin C deficiency in different tissues and organs of juvenile C. carpio var. Jian.