细胞骨架蛋白destrin在创伤性动脉瘤血管壁组织中的表达及其临床相关性研究

Expression of destrin in vessel wall tissue of traumatic arterial aneurysm and study of its clinical relativity

[目的]研究细胞骨架蛋白中destrin蛋白在创伤性动脉瘤血管壁组织中的表达情况及其与创伤性动脉瘤临床表现及病理特征等的关系,分析其在血管壁组织修复过程中的可能作用。[方法]采用Western-blot免疫印迹分析和实时荧光定量(RT-PCR)方法检测destrin蛋白在13例创伤性动脉瘤血管壁组织和8例正常人股动脉组织中的定量表达,比较其蛋白表达差异,并将结果与临床资料进行统计学分析,进行蛋白表达失衡与临床相关性研究。[结果]细胞骨架蛋白destrin在创伤性动脉瘤血管壁组织中的表达明显低于正常人胸主动脉组织,比较有统计学差异(P<0.05)。destrin的蛋白表达水平变化与吸烟显示相关,与年龄、受损动脉直径、高血压、术中发现血栓化程度等临床参数未见明显相关。[结论]细胞骨架蛋白destrin在创伤性动脉瘤血管壁组织中的表达相较正常人股动脉组织下调,该蛋白表达水平与创伤性动脉瘤临床相关参数(吸烟)有关。该研究为探讨TAA的潜在分子机理与防治措施奠定了初步依据。

[ Objective] To investigate the expression of protein destrin in cytoskeleton protein in traumatic arterial aneurysm （TAA） vessel wall tissue and its relationship with clinical representations and pathological characteristics. [ Method] Western - blot analysis and RT - PCR were used to detect the quantitative expression of destrin protein in 13 patients with TAA and in the femoral arterial tissues of 8 normal subjects. The difference of protein expression was compared ,＇and the results with clinical data were analyzed statistically. The correlation of unbalanced expression of destrin protein with the clinical presentations was studied. [ Result] The expression of destrin in TAA tissue was lower than that in the femoral artery of normal subjects （ P 〈 0. 05） . The protein expression of destrin was correlated with smoking but not correlated with clinical parameters such as age, diameter of artery, hypertension, and intraoperative thrombus degree. [ Conclusion] The expression of protein destrin in TAA tissue is lower than that in the femoral artery of normal subjects. The protein expression is correlated with smoking. The study may be helpful for the further investigation of the molecular mechanisms involved in TAA.