丙氨瑞林对卵巢癌细胞增殖及凋亡的影响

Effects of Alarelin on Proliferation and Apoptosis in Ovarian Cancer Cell Line CoC1/cDDP

目的:探讨丙氨瑞林对卵巢癌细胞株CoC1/cDDP增殖及凋亡的影响及其作用机制。方法:将丙氨瑞林与CoC1/cDDP一起培养,采用四甲基偶氮唑蓝(MTT)比色法检测细胞增殖抑制率,流式细胞仪进行细胞周期时相及凋亡分析,瑞氏-姬姆萨染色后观察凋亡细胞形态,放射免疫法测定培养液中CA125的变化,半定量RT-PCR法检测作用前后CoC1/cDDP细胞Survivin-ΔEx3及Caspase-3 mRNA的表达。结果:(1)随着丙氨瑞林浓度的升高,作用时间的延长,细胞生长抑制率逐渐上升(P<0.01)。(2)丙氨瑞林作用后的CoC1/cDDP细胞,G0/G1期细胞比例增加,S期及G2/M期细胞比例减少,细胞增殖指数(PI)降低(P<0.01);培养液中CA125的水平下降,与PI正相关(r=0.893,P<0.05);并且这些变化与丙氨瑞林的浓度相关。(3)作用后亚G1期及Annexin V+/PI-细胞的百分率较对照组明显升高(P<0.01),并出现明显的凋亡细胞形态的改变;Survivin-ΔEx3 mRNA表达与对照组比较无明显变化(P>0.05),而Caspase-3 mRNA的表达上调,并随着丙氨瑞林浓度的升高而表达增加(P<0.01),且与亚G1期及Annexin V+/PI-细胞的比例呈正相关(r分别为0.994及0.978,P<0.01)。结论:丙氨瑞林能直接抑制卵巢癌细胞株CoC1/cDDP细胞的增殖,并诱导其凋亡,作用机制与丙氨瑞林上调Caspase-3 mRNA的表达有关。

Objective： To investigate the effects and mechanism of Alarelin on proliferation and apoptosis in the drugresistant human ovarian cancer cell line CoC1/cDDP in vitro, Methods： CoC1/cDDP cells were treated by different concentrations of Alarelin in different time courses. Inhibition of cell proliferation was tested by MTT assay. The cell cycle, sub-G1 and Annexin V^＋/PI^- cell content were analyzed by flow cytometry （FCM）. The morphologic changes of CoG 1/cDDP cells were observed by Wright staining. CA125 level in the culture medium was measured by RIA. The expression of Survivin-AEx3 mRNA and Caspase-3 mRNA were measured by semi-quantitative RT-PCR. Results： （1）Alarelin inhibited CoG1/cDDP cell proliferation significantly in a dose-dependent and time-dependent manner（P 〈 0.01 ）. （2）After treated by Alarelin,the number of CoC1/cDDP cells in G0/G1 phase was elevated,the numbers of CoC1/cDDP cells in S and G2/M phase were obviously decreased, and the proliferation index （PI）of CoG 1/cDDP was decreased （P 〈 0.01 ）. The level of CA125 in the culture medium was decreased and had a positive correlation with the PI （r = 0.893,P〈 0.05）. （3） COG1/ cDDP cells appeared the classical apoptotic morphology, the percentages of sub-G1 and Annexin V^＋/PI^- cells were increased （P 〈 0.01 ）. The expression of Caspase-3 mRNA was increased dose-dependently and had a positive correlation with the apoptotic rate （r = 0.994,r = 0.978,P 〈 0.01 ）. There was no change in the expression of the Survivin-AEx3 mRNA （P 〉 0.05）. Conclusion： Alarelin can inhibit proliferation and induce apoptosis of the drug-resistant human ovarian cancer cell line CoC1/cDDP as well. This effect may be related to up-expression of Caspase-3 mRNA by Alarelin.