人参总皂苷对戊酸雌二醇诱导的多囊卵巢大鼠神经生长因子表达的影响

Effects of ginseng total saponins on nerve growth factor expression in rat with estradiol valerate-induced polycystic ovaries

目的:探讨人参总皂苷(ginseng total saponins,GTS)对多囊卵巢(polycystic ovary,PCO)大鼠神经生长因子(nerve growth factor,NGF)表达的影响。方法:30只成熟雌性SD大鼠,随机分为正常对照组、模型组和GTS组,每组10只。模型组和GTS组大鼠一次性肌肉注射戊酸雌二醇(estradiol valerate,EV)4mg诱导PCO。GTS组大鼠于造模后第2天开始隔日1次腹腔内注射GTS50mg/kg,共30d。免疫组织化学法检测大鼠下丘脑、垂体和卵巢组织中NGF表达。结果:模型组大鼠下丘脑、垂体和卵巢中NGF的表达高于正常组(P<0.05)。与模型组比较,GTS组大鼠卵巢中NGF表达较低(P<0.05),而下丘脑和垂体中NGF表达无明显变化。结论:GTS可能通过降低卵巢组织中NGF的表达治疗多囊卵巢大鼠。

Objective： To investigate the effect of ginseng total saponins （GTS） on expression of nerve growth factor in rat with polycystic ovaries. Methods： A total of thirty rats were randomly divided into normal control group, untreated group and GTS group. Polycystic ovaries were induced by a single intramuscular injection of 4 mg estradiol valerate （EV） in rats of the untreated group and GTS group. The rats in the GTS group were administered 50 mg/kg GTS every other day by intraperitoneal injection for 30 days after the EV injection. The expressions of NGF in the ovaries, pituitary and hippocampus were observed by method of immunohistochemistry. Results： Comparison with the normal control group revealed that the expressions of NGF in ovaries, pituitary and hippocampus of rats in the untreated group were increased （ P〈0.05）. The ovarian morphology of the GTS group was almost as normal as that of the normal control group. As compared with the untreated group, the expression of NGF in ovaries of the rats in GTS group was obviously decreased （ P〈0. 05）, while the expression of NGF in pituitary and hippocampus showed on difference. Conclusion： GTS can decrease the expression of NGF in ovary tissue in rats with polycystic ovaries induced by EV, which may be its mechanism in lessening the polycystic ovary.