补肾健脾活血方对兔胫骨应力性损伤的防治作用

Bushen Jianpi Huoxue Recipe in prevention and treatment of tibial stress injuries in rabbits

目的:探讨补肾健脾活血方对兔胫骨应力性损伤的防治作用。方法:雄性成年兔35只,随机分为安静对照组、跑跳运动1周组、跑跳运动2周组、跑跳运动3周组、跑跳运动4周组、服药1周运动组和服药2周运动组,每组各5只。采用自制高电压、低电流刺激仪刺激实验兔进行被动跑跳运动。观测实验兔胫骨X线表现、核素骨显像、组织学与电镜变化,采用放射免疫法检测血清甲状旁腺素(parathyroid hormone,PTH)、骨钙素(bone Gla protein,BGP)和睾酮的含量。结果:持续刺激2周后,骨细胞呈现应力性损伤的趋势,血BGP和血PTH均显著升高,血睾酮显著降低。持续刺激3～4周后,X线及核素骨显像均表现阳性,组织学及血生化出现应力性损伤的变化。两服药组X线及核素骨显像均呈阴性,皮质内出现新生骨,骨细胞具有较典型的成骨相,血PTH呈下降趋势,血BGP和血睾酮水平均显著高于同周运动组。结论:补肾健脾活血方药具有明显刺激新骨形成,提高血睾酮水平的作用,对胫骨应力性损伤起到较好的防治作用。

Objective： To explore the effects of Bushen Jianpi Huoxue Recipe, a compound traditional Chinese herbal medicine for tonifying the kidney, invigorating the spleen and promoting blood circulation, on tibial stress injuries in rabbits. Methods： Thirty-five mature male rabbits were used in the experiment, and randomly divided into 7 groups： sedentary control （SC） group, 1-week exercise （E-1W） group, 2-week exercise （E-2W） group, 3-week exercise （E-3W） group, 4-week exercise （E-4W） group, 3-week exercise and 1-week Chinese herbal medicine treatment （EMT-1W） group, and 4-week exercise and 2-week Chinese herbal medicine treatment （EMT-2W） group. There were 5 rabbits in each group. A rabbit model of tibial stress injuries was established by stimulating the rabbits to jump and run within a high-voltage and low-current electronic cage. Radiologic features, bone scintigraphy, histology and electron microscopy of rabbit tibia were observed, and the content of blood parathyroid hormone （PTH）, bone gla protein （BGP） and testosterone was detected by using radioimmunoassay method. Results： After 2-week exercise, changes in histology and osteocytes had a tendency towards stress injuries. Serum PTH and BGP levels were remarkably increased, while serum testosterone level was lower than that in the SC group. Three- and four-week continuous exercise resulted in tibial stress injuries, and the positive changes were observed in X-ray features and radionuclide images. Compared with E-3W group, the levels of serum BGP and testosterone were decreased remarkably. A series of positive results such as prevailing negativeness of X-ray features and radionuclide images, increasing process of osteogenesis, typical osteogenic phase of osteocytes and favorable transformation of biochemical markers was shown in EMT-1W and EMT-2W groups, It also showed remarkable rising levels of serum BGP and testosterone and remarkable reducing level of serum PTH in EMT-1W and EMT-2W groups as compared with E-1W and E-2W groups. Conclusion： Bushen Jianpi Huoxue Recipe can prevent and treat exercise-induced tibial stress injuries by stimulating new bone formation and increasing serum testosterone level.