摘要
目的:研究COX-2在喉鳞状细胞癌中的表达,探讨其与喉癌的生长、浸润及转移的关系。方法:应用半定量RT-PCR方法对标本中COX-2 mRNA的表达进行检测,应用免疫组织化学方法对标本中COX-2蛋白的表达进行检测,并对COX-2的表达情况与疾病生物学特点的关系进行研究。结果:COX-2 mRNA及蛋白在喉鳞状细胞癌组织中高表达,与在声带息肉组织中的表达差异有统计学意义(P<0.01),且随肿瘤的进展COX-2的表达强度增加。结论:COX-2是较为有效的生物学指标,指示喉鳞状细胞癌的发生和发展。同时应用RT-PCR方法及免疫组织化学方法检测喉癌标本,既保证了灵敏度又兼顾了特异度。
Objective：To investigate the expression of COX-2 in both molecule and protein levels in laryngeal squamous cell carcinoma and the relationship between its intensity and histological grade, clinical stage and lymphoma metastasis. Method： By using emi-quantify RT-PCR and Immunohistochemistry, the expression of the COX- 2 mRNA and COX-2 protein in laryngeal squamous cell carcinoma and polyp of vocal cord were examined. Result： Compared with the polyp of vocal cord tissue, the expression level of COX-2 mRNA and COX-2 protein in human laryngeal squamous cell carcinoma were significantly increased （P〈0.01）. The intensity of COX-2 mRNA and protein expression increased as the laryngeal squamous cell carcinoma progresses developed. Conclusion： COX-2 is high expressed in human laryngeal squamous cell carcinoma, and it may play an significant role in the growth, invasion and metastasis of human laryngeal squamous cell carcinoma. A combination of emi-quantify RT-PCR and immunohistochemisty is sensitive and specific for laryngeal neoplasms detection.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2008年第12期532-535,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
