环介导等温扩增快速检测EBV DNA对鼻咽癌诊断的意义

The value of loop-mediated isothermal amplification method for rapid diagnosis of EBV DNA

目的:建立环介导等温扩增技术快速检测EB病毒的方法,为鼻咽癌的早期诊断提供辅助手段。方法:利用煮沸法快速提取样品中病毒DNA,使用环介导等温扩增技术检测EB病毒特异基因片段,在等温条件下于1 h内完成检测过程。结果:环介导等温扩增技术在对33份临床标本的检测中显示了较高的特异性,较现有的PCR技术更加简便快速,能在等温条件下进行,不需复杂的仪器设备,检测成本大大降低。结论:本研究为鼻咽癌临床检测提供了一个快速简便的新辅助方法。

Objective：To establish a rapid method for EBV detection with loop-mediated isothermal amplification （LAMP）, and to make it as a clue for early diagnosis of nasopharyngeal carcinoma cancer. Method： EBV DNA was fast extracted from samples after boiling, while the whole detection will be finished within an hour with specific amplification of EBV gene by LAMP. Result： High specificity was shown from EBV detection of 33 clinical samples. Comparing with PCR, LAMP is more simple and convenient to perform under isothermal conditions, and require no special apparatus, thus, it is more economical and practical. Conclusion： LAMP analysis of EBV may be an efficient and easy way for clinical diagnosis of nasopharyngeal carcinoma.