白藜芦醇对小鼠离体心脏负性变时作用机制的研究

Study on the action mechanism of resveratrol on isolated heart negative chronotropic effect

目的观察白藜芦醇(resvaratrol,Res)对小鼠离体心脏自律性的影响。方法测定Res对离体小鼠心率(HR)影响的量效曲线,测定不同类型钾通道阻断剂对Res产生负性变时作用的影响。结果Res可减慢HR(P<0·05);与Res组相比,格列苯脲(Glibenclamide,Gli)组、四乙胺(tetraethylammonium,TEA)组可部分阻断Res的作用(P<0·05),4-氨基吡啶(4-aminopyridine,4-AP)组、氯化钡(barium chloride,BaCl2)组、阿托品(Atropine)组不能阻断Res的作用(P>0·05)。结论Res可呈剂量依赖性减慢HR,其作用机制与开放ATP敏感性K+通道(KATP)及Ca2+依赖性K+通道(KCa)有关。

Objective To observe the effect of resvaratrol （Res） on the automaticity of isolated heart. Methods The quantityeffect curve of Res＇ effect on heart rate （HR） of isolated heart of the mice was determined to detect the effect of different types of K channel blockers on Res induced isolated heart negative chronotropic effect. Results Res decreased HR （ P 〈 0. 05 ） ; glibenclamide and tetraethyl- ammonium groups partly blocked Res＇ effect（ P 〈 0. 05 ） , but 4-aminopyridine, barium chloride and atropine groups didn＇t block Res＇ effect （ P 〉 0. 05 ）. Conclusions Res could decrease HR with dosage dependency, its action mechanism might be related to opening ATP sensitivity K＋ channel（KATp） and Ca2＋ dependent K＋ channel（Kca）.