鼻息肉基因芯片检测及基因表达谱的研究

Gene expression profiles in human nasal polyps studied by DNA microarray

目的:应用寡聚核苷酸基因表达谱芯片研究鼻息肉(NP)基因表达谱的变化。方法:应用包含14500条人类全长基因的寡聚核苷酸芯片HG-U133A2.0检测6例NP组织,6例正常鼻黏膜组织的基因表达谱,分析差异表达基因,并选择其中有差异表达的部分基因进行realtime RT-PCR验证。结果:基因芯片筛选出大量差异表达的基因,其中包括炎性反应、免疫应答、免疫调控及信号传导的相关基因及其受体,如IL-8、RGS1、GRK4、CCL20、子宫球蛋白(uteroglobin)等。实时荧光定量PCR测定差异表达基因IL-8、RGS1与基因芯片结果一致。结论:NP基因表达谱存在差异,RGS1可能通过影响细胞信号传导通路发挥作用,IL-8可能通过促使炎性细胞释放多种炎性递质参与鼻息肉发病。

Objective：To explore the changes in gene expressions in the human nasal polyp.Method：A 14500 gene DNA microarray （Affymetrix） was used to examine gene expressions in 6 NP samples,6 normal mucosal samples.The differentially expressed genes were identified and subjected to realtime PCR analysis.Result：The differentially expressed genes mostly involved in cytokines ,adhesion genes about complements and their receptors,immune transcription regulatory molecules, signal transduction, differentially expressed genes including IL-8,RGS1,GRK4,CCL20, uteroglobin. The results of IL-8、RGS1Real-time PCR of were consistent with that of gene chip analysis.Conclusion：Microarray expression profile of NP samples is differential. RGS1 may play an important role in cell signal transduction, IL-8 may involve in occurrence of nasal polyps by inducing cells releasing inflammatory factors.