rhIL-6促进致死性辐射损伤小鼠凝血及早期造血功能的恢复

Effects of rhIL6 on recovery from radiationinduced hemopoietic aplasia in mice

目的：观察ｒｈＩＬ－６对小鼠造血功能的影响。方法：应用致死性６０Ｃｏ－γ射线辐射Ｂａｌｂ／ｃ小鼠，造成骨髓型辐射损伤模型。照射后用ｒｈＩＬ－６皮内注射给药６ｄ（２μｇ／只，２次／ｄ），通过检测出凝血时间（ＢＴ，ＣＴ）、凝血酶原时间（ＰＴ）、外周血ＰＬＴ和ＷＢＣ，以及骨髓造血细胞体外培养ＣＦＵ－Ｍｉｘ和脾脏ＣＦＵ－Ｓ的数目，研究了ｒｈＩＬ－６对骨髓型辐射损伤小鼠早期造血功能恢复的效应，并进行了机理的探讨。结果：ｒｈＩＬ－６处理组小鼠的ＢＴ，ＣＴ和ＰＴ均较对照组显著缩短（Ｐ＜０．０１）；外周血ＰＬＴ和ＷＢＣ分别较对照组提高１３０％和１６５％。骨髓造血细胞体外培养ＣＦＵ－Ｍｉｘ和脾脏ＣＦＵ－Ｓ计数，均显著高于对照组（Ｐ＜０．０１）。结论：ｒｈＩＬ－６具有促进受辐射小鼠早期造血细胞恢复的生物学效应，可减轻辐射所致骨髓损伤和促进造血干／祖细胞（ＨＳＣ／ＨＰＣ）的增殖、分化，有利于骨髓造血功能的恢复。

Using highly expressed rhIL6 E.coli, and of Sephadex G50, which gains more than 95% purity and 4.83 × 1011 u/g contrast activity, the effects of rhIL6 on hematopoiesis of murine was observed. Balb/c mice received a lethal dose irradiation of 60Coγ to establish a model of acute bone marrow type radiation injury. After received radiation, subcutaneous injection of rhIL6 was given for 6 days(2 μg, twice a day). By observing changes in BT, CT, PT, peripheral PLT and WBC, CFUMix cultured in vitro and CFUS in spleen, analysis was thus made on the effects of rhIL6 on the recovery from radiationinduced hematopoietic aplasis in order to study its mechanisms. Results show that the BT, CT and PT of the rhIL6 treatment group was remarkably shorter than the control group(P<0.01). The peripheral PLT and WBC were increased to 130% and 165%. The CFUMix cultured in vitro and CFUS were markedly higher than the control group(P<0.01) after H test. This research suggests that rhIL6 exerts biological effects on the hematopoietic cells of radiationinduced mice, can attenuate radiation injury and stimulate the proliferation and differentiation of hematopoietic stem/progenitor cells, which helps hematopoietic recovery of bone marrow.