rhIL-1β对四氧嘧啶型糖尿病大鼠胰腺损伤的保护作用

Protective effect of rhIL1β on pancreatic islets of alloxaninduced diabetic rats

采用ＡＢＣ免疫组织化学染色及体视学技术，观察了白介素－１β（ｒｈＩＬ－１β）对四氧嘧啶引起的大鼠胰腺Ｂ细胞损伤的保护作用。皮下注射四氧嘧啶（１５０ｍｇ／ｋｇ）的大鼠４８ｈ后，其血浆胰岛素浓度显著下降，血糖浓度显著升高，ＡＢＣ免疫组织化学染色及体视学技术测得其胰岛素免疫反应阳性胰岛的数密度和体密度均为０。预先皮下注射ｒｈＩＬ－１β的大鼠在皮下注射四氧嘧啶（１５０ｍｇ／ｋｇ）４８ｈ后，尽管其血浆胰岛素浓度显著下降，但其血糖浓度与正常对照组相比升高不明显。与四氧嘧啶组相比较，其血糖水平显著下降，ＡＢＣ免疫组织化学染色及体视学技术测得其胰岛素免疫反应阳性胰岛的数密度和体密度值，均显著高于四氧嘧啶组。本文结果提示，ｒｈＩＬ－１β预处理对四氧嘧啶型糖尿病大鼠的胰腺损伤具有一定的保护作用。

Protection of rhIL1β for pancreatic islets of alloxaninduced diabetic rats was demonstrated with methods of immunocytochemistry and stereology. The concentration of serum glucose increased but that of insulin decreased after administration of alloxan( 150 mg/kg), and the volume density and numerical density of the islets were zero. In rhIL1β pretreated rats, although the concentration of serum insulin decreased, that of glucose was at normal level compared with the control group. As compared with alloxan group, the concentration of serum glucose in rhIL1β pretreated rats decreased and the volume density increased. The above results suggest that pretreated rhIL1β may have protective effect on the islets of alloxaninduced diabetic rats.