MUC2诱导抗肿瘤免疫反应的实验研究

Experimental study of antitumor immune response induced by MUC2

目的：探讨肿瘤粘液糖蛋白的核心多肽ＭＵＣ２诱导的抗肿瘤免疫应答。方法：测定多肽ＭＵＣ２免疫后小鼠的皮肤迟发型超敏反应（ＤＴＨ）和脾淋巴细胞的体外细胞毒反应。结果：ＭＵＣ２可诱导小鼠的ＤＴＨ；体外脾淋巴细胞对ＭＵＣ２表达阳性的粘液腺癌细胞系，如人胰腺癌细胞株ＳＷ１９９０和人胃癌细胞株ＫＡＴＯ－３，都具有强的细胞毒作用；抗ＭＵＣ２的抗体ＬＳＢ可抑制体外鼠脾细胞的细胞毒作用。结论：肿瘤相关抗原ＭＵＣ２可诱导小鼠的细胞免疫应答，并可能作为一种有效的免疫原用于提高抗肿瘤免疫应答。

Aim: To investigate the antitumor immune response induced by MUC2. Methods: Delayed type hyperactivity(DTH) in mice and cytotoxic activity of MUC2activited spleen cells were investigated in vitro. Results: MUC2specific DTH response could be elicited by MUC2, the cytotoxicity of activited spleen cells in vitro was MUC2 specific. Conclusion: MUC2 as an important tumor associated antigen can induce cellular immune response and may be an effective immunogen to augment the antitumor immune response.