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HIV核心抗原p24检测方法的建立及应用 被引量:3

Establishment and application of a sandwich ELISA assay for HIV p24
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摘要 HIV感染的检测在预防AIDS传播方面有重要作用。本研究以重组抗原pG1免疫Balb/c小鼠,应用淋巴细胞杂交瘤技术建立了5株识别HIV核心抗原p24的杂交瘤细胞株D3,1H1,2G10,3H5和4H6。经鉴定这5株McAb与其它病毒抗原无交叉反应,分别识别3个不同的抗原表位。应用两株识别不同表位的McAb2G10和3H5建立了检测HIVp24抗原的夹心ELISA法,并初步检测了24份HIV抗体阳性血清。 Recombinant protein pG1 containing total sequence of HIV core antigen p24 was used as an immunogen to immunize Balb/c mice and five hybridomas secreting McAbs to p24, D3, 1H1, 2G10, 3H5 and 4H6, were raised by conventional B lymphocyte hybridoma technique. The Ig subclass, recognized epitope and specificity of obtained McAbs were identified. The results showed that these five McAbs recognized three different epitopes of p24 molecule respectively and did not crossreact with HBcAg, HCV C region and NS3 region and E.coli protein. Using two McAbs recognizing two distinct epitopes, a sandwich ELISA method for measurement of HIV p24 was established with sensitivity about 1 ng/ml.
出处 《细胞与分子免疫学杂志》 CAS CSCD 1997年第4期51-54,共4页 Chinese Journal of Cellular and Molecular Immunology
关键词 HIV P24 单克隆抗体 ELISA AIDS HIV p24 McAb ELISA
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参考文献4

  • 1刘荷中,国外医学流行病学、传染病学分册,1996年,23卷,3期,126页
  • 2韩保光,中华微生物学和免疫学杂志,1996年,16卷,4期,239页
  • 3鄂征,组织培养和分子细胞学技术,1995年,419页
  • 4吴卫星,中华流行病学杂志,1992年,13卷,特刊2期,320页

同被引文献25

  • 1刘荷中.HIV核心抗原p24检测技术及其应用的研究进展[J].细胞与分子免疫学杂志,1996,12(1):59-64. 被引量:2
  • 2金冬雁 黎孟枫 等.分子克隆(第二版)[M].北京:科技出版社,1992.363.
  • 3Hnrard DR, Wu S, Phillips J,et al. Detection of p24 antigen with and without immune complex dissociation for longitudinal monitoring of human immunodeficiency virus type 1 infection. J Clin Microbiol,1995,33(1):72
  • 4barb F,Klein M,Badonel Y. Early detection of antibodies to human immunodeficiency virus 1 by a third generation enzyme immunoassay. A comparative study with the results of second generation immunoassay and western blot .Ann Biol Clin(Paris),1994,52(5):341
  • 5Alan JS,Coligan JE,Lee TH,et al. A new HTLV-/LAV encoded atigen detected by antibodies from AIDS patients.Science,1985,230:810
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  • 7Weber bernard,Fall EL H M,Berger A, et al. Reduction of dagnostic window by New Fourth-Generation human immunno-deficiency virus screening assays. J Clin Microbiol,1998, 36(8):2235
  • 8Blandine JA. B cell epitopes of HIV type 1 p24 capcid protein:a reassessment. AIDS and Human Retroviruse,1996,12:519
  • 9Safe blood and product MODULE2. Screening for HIV andother infections agent.中文版,WHO/GPA/CNP/93.2
  • 10金冬雁(译),分子克隆(第2版),1992年,860页

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