X-刀治疗后脑胶质瘤中PCNA和p53基因表达的变化及意义

Changes of p53 and PCNA Gene Expression in Human Gliomas After Xknife Treatment

为了探讨经Ｘ－刀治疗后脑胶质瘤中ＰＣＮＡ和ｐ５３基因表达的变化，作者采用组织免疫组化ＡＢＣ法，检测３６例经Ｘ－刀治疗后脑胶质瘤和３０例对照组的标本，分别计算增殖细胞核抗原标记率（ＰＣＮＡ－ＬＩ）和ｐ５３基因表达标记率（ｐ５３－ＬＩ）。结果表明，治疗组的ＰＣＮＡ－ＬＩ明显低于对照组（Ｐ＜０．０５），治疗组的ｐ５３－ＬＩ明显高于对照组（Ｐ＜０．０５）；治疗组和对照组内ＰＣＮＡ与ｐ５３基因的表达均呈显著性正相关关系。作者认为，ＰＣＮＡ和ｐ５３基因的表达可作为脑胶质瘤对放射敏感性和恶性程度的标志之一。

In order to investigate the changes of PCNA and p53 gene expression after Xknife treatment,Immunohistochemistry technique (ABC method)was employed in the specimens of 36 cases of brain glioma after Xknife treatment and 30 cases of control. PCNALI and p53LI were calculated respectively. PCNALI in treatment group was lower than in control group. p53LI in treatment group was higher than in control. PCNA was positively significantly correlated with p53 gene expression both in the treatment group and control group. The authors suggest that PCNA and mutant p53 gene expressions might be a symbol of radiation sensitivity and malignant degree.