晚期血吸虫病患者肝内T细胞亚群的免疫组织化学检测

Immunohistochemical Detection of T Cell Subset in the Liver of Patients with Advanced Schistosomiasis Japonica

采用免疫组织化学技术检测了22例晚期血吸虫病（下称晚血）患者肝内T细胞亚群。发现晚血患者肝内T细胞可集中分布于虫卵肉芽肿外层或者散在分布于非肉芽肿部位的虫卵附近，广泛纤维化的区域无T细胞浸润。肉芽肿和非肉芽肿部位的T细胞均以CD8+T细胞（抑制性／细胞毒T细胞）为主。晚血患者肝脏病变以汇管区纤维化为主，浸润细胞不多，仅27．27％（6／22）患者肝内存在已被免疫调节了的慢性虫卵肉芽肿。提示晚血患者肝内细胞免疫反应很弱，肝内的CD+8T细胞可能主要是抑制性T细胞（Ts）。

The T cell subset in the liver of 22 patients with advanced schistosomiasis japonica weredetected with immunohistochemical technique. It was found that T cells, were majorly distributed in theoutlayer of egg granulomas or near the eggs in the non-granuloma areas, but no T cell was infiltrated in the areas ofextensive fibrosis in the liver of patients with advanced schistosomiasis japonica. The T Cells in bothgranuloma and non-granuloma areas were mostly CD8 T cells (suppressive/cytotoxic T cells, Ts/Tc). The mainpathological change in the liver of patients with advanced schistosomiasis japonica was portal fibrosis with onlyrare infiltration of cells. The immunoregulated and chronic egg granulomas were observed only in the liver of27. 27% (6/22) of patients with advanced schistosomiasis japonica. The results indicate that the cellularinmune response is very weak in the liver of patients with advanced schistosomiasis japonica. The CD8 T cells inthe liver of patients with advanced schistosomiasis japonica may be mainly suppressive T cells.