新的癫痫相关基因的筛选及部分cDNA序列的分离鉴定

Screening of Epilepsy-related Genes and Isolation and Identification of cDNAs

用mRNA差异显示技术从大鼠癫痫状态的海马分离了20个cDNA差异片段，对4个cD-NA片段进行序列分析并在基因库中进行相同性比较，发现ERG8、ERG11、ERG12无相同性序列，ERG14与鼠的微管相关蛋白基因有64％～69％的相同性。由于这些基因的差异表达是致痫剂马桑内酯和抗痫剂MK－801所致，所以，ERG8可能是新的候选癫痫基因，ERG11和ERG12可能是新的候选抗痫基因；由于微管相关蛋白高表达与惊厥条件下海马苔藓纤维侧枝出芽关系密切，因此，ERG14在癫痫早期高表达可能预示着新的轴突生长和突触形成。

20 cDNA differential fragments were isolated from the hippocampus of rat in epileptic stateusing mRNA differential display technique. 4 fragments were sequenced and compared with the knownsequences in the Genebank, which showed that ERGS, ERGll, ERG12 had no significant identity to anyknown sequences, ERG14 had 64%～69% identity to microtubulin-associated protein of rat. Because thedifferential expressions of these genes were caused by epilepsy inducer Coriaria lactone and anti-epilepsy drugMK-801 and ERGS may be a novel candidate epilepsy gene, ERG11 and ERG12 may be novel candidateantiepilepsy genes. Since the microtubulin-associated protein is closely associated with the collateral sprouting ofmossy fibers in the hippocampus of seizured rat, the high expression of ERG14 in the early stage of epilepsymay predict the growth of axon and formation of synapse.