摘要
利用化学发光分析技术建立了一种测定体液中腺苷脱氨酶活性的新方法，该方法简便易行，快速灵敏。对酶反应最佳条件进行了探讨，底物Km为0．31mmol／L，最佳pH为7．40，反应时间为10min。该方法的重复性CV为2．74％。该方法对结核性胸膜炎和癌性胸水的鉴别诊断有实用价值。
A novel analytical method of determining the activity of adenosine deaminase(ADA) in bodyfluids by luminescent assay was described in this paper. This method is simple,rapid,sensitive and accurate.Optimum reaction conditions for activity of ADA were investigated. The substrate Km was 0. 31 mmol/L.The optimum PH was 7. 40. The reaction time was 10 minutes. The repetition of this method was 2. 74%and results from luminescent assay were significantly related to results from colormetric assay. Theluminescent method can be effectively applied in discrimination between tuberculous pleuritis and cancerous pleuritis.
出处
《同济医科大学学报》
CSCD
1997年第6期438-441,共4页
Acta Universitatis Medicinae Tongji
