健脾益气方对大鼠急性胃粘膜损伤预防作用的实验研究

Experimental Study on Protective Effects of Jianpiyiqi Prescription on Acute Gastric Mucosa Injury in Mice

采用束缚水浸法复制大鼠急性胃粘膜损伤模型，观测溃疡指数，检测血浆及胃粘膜组织中前列腺素E2（PGE2），利用Westerndotblot法检测血浆及胃组织中热休克蛋白（HSPs）；并对胃组织细胞HSPs分布进行免疫组织化学研究。结果发现：健脾益气方（JPYQ）组和热处理组的溃疡指数极其显著低于模型组（P＜0．01），但血浆及胃组织中PGE2水平明显高于模型组（P＜0．05），同时，血浆及胃组织中HSPS水平也明显高于模型组（P＜0．05）。提示JPYQ及热处理能抑制大鼠血浆及胃组织中PGE2水平下降，并诱导大鼠提高HSPs的表达水平，从而预防胃粘膜损伤。

The mouse model of acute gastric mucosa injury was established by using tying waterimmersion to determine the ulcer index,levels of PGE2 in serum and the tissues of gastric mucosa. The content ofheat shock proteins (HSP2) in serum and the tissues of gastric mucosa was examined by using Western dotblot method and the distribution of HSPs in the cells of gastric mucosa was immunohistochemicallyinvestigated. Our results showed that the ulcer indices in Jianpiyiqi group (JPYQ group) and heat treated group werestatistically lower,the level of PGE2 and the content of HSPs in serum and the tissues of gastric mucosasignificantly higher than those in model group (P<0. 01, <0. 05, <0. 05, respectively). The results suggestthat JPYQ prescription and heat treatment can inhibit the decrease of the levels of PGE2 in serum and thetissues of gastric mucosa,and induce the elevation of the expression level of HSPs in mice, thereby protectinggastric mucosa from injury.