TDZ和GA_3对提高红叶石楠组培快繁效率的研究

Effectiveness of TDZ and GA_3 on tissue culture and rapid propagation of Photinia Fraseri

研究苯基噻二唑(TDZ)和赤霉素(GA3)对红叶石楠组培快繁效率的影响,结果表明:红叶石楠两品种继代培养能适应多种基本培养基,在MS、4/5 MS和B53种基本培养基中,红叶石楠的继代增殖率均达到3.0以上;在继代培养中,TDZ、GA3的添加对继代周期、年增殖率均有显著影响,但对增殖倍率没有显著影响;在生根培养基中添加GA3有效地缩短生根诱导时间及生根培养时间,提高红叶石楠的组培快繁效率,降低组培苗的生产成本。

Subculture cycle, proliferation rate, annual proliferation coefficient, roonng rate, rooting-culture time factors were studied to evaluate the effect of TDZ and GA3 on stem tissue culture and rapid propagation of Photinia fraseri. The results showed that several basic media were suitable for the proliferation culture of the 2 varieties ofP. fraseri. In 3 media, i. e. , MS, 4/5MS and B5, the proliferation rates were greater than 3.0. Among the subcultures, addition of TDZ and GA3 had a significant effect on both subculture cycle and annual proliferation coefficient, but no significant effect on proliferation rate. On the rooting culture, the supplement of GA3 shortened the root-inducing and rooting-culture time, improved micropropagation efficiency for P. fraseri, and reduced the cost of seedlings by tissue culture.