摘要
位点特异性重组系统能够在植物遗传转化中更准确、可靠的操纵外源DNA的引入或删除,已成为植物遗传操作中的重要工具。本文简要介绍目前应用最为广泛的Cre/loxp位点特异性重组系统的重组机制,并着重阐述Cre/loxp系统在删除转基因植物中标记基因及定点整合等方面的应用。
Site-specific recombination technology has been developed and widely applied for plant genetic manipula tions. It can successfully delete marker genes from a transgenic plant or site-specifically integrate a gene to a pre-determined genomic location in a precise, single-copy pattern. This article reviews the Cre/loxp site-specific recombi nation technique and its applications for marker gene deletion and precise gene integration.
出处
《福建农业学报》
2008年第2期211-217,共7页
Fujian Journal of Agricultural Sciences
基金
福建省自然科学基金(B01220003)