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Cre／loxp位点特异性重组系统在转基因植物中的应用 被引量：4

Application of Cre/loxp site-specific recombination technology on transgenic plants
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摘要 位点特异性重组系统能够在植物遗传转化中更准确、可靠的操纵外源DNA的引入或删除,已成为植物遗传操作中的重要工具。本文简要介绍目前应用最为广泛的Cre/loxp位点特异性重组系统的重组机制,并着重阐述Cre/loxp系统在删除转基因植物中标记基因及定点整合等方面的应用。 Site-specific recombination technology has been developed and widely applied for plant genetic manipula tions. It can successfully delete marker genes from a transgenic plant or site-specifically integrate a gene to a pre-determined genomic location in a precise, single-copy pattern. This article reviews the Cre/loxp site-specific recombi nation technique and its applications for marker gene deletion and precise gene integration.
作者 邱淑萍 陈在杰 王锋
机构地区 福建省农业科学院福建农业遗传工程重点实验室
出处 《福建农业学报》 2008年第2期211-217,共7页 Fujian Journal of Agricultural Sciences
基金 福建省自然科学基金(B01220003)
关键词 位点特异性重组 Cre/loxp 转基因植物 基因删除 定点整合 site-specific recombination Cre/loxp transgenic plant gene deletion site-specific integration
分类号 Q943.2 [生物学—植物学]
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参考文献47

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二级参考文献54

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福建农业学报
2008年 第2期

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