规模化养殖场大肠杆菌的分离鉴定与耐药性监测

Identification and Drug Resistance Surveillance of Escherichia coli in Large Scale

从武汉郊区规模化养殖场采集腹泻仔猪的棉拭子和病死动物的泄殖腔分泌物或肝脏,分离鉴定出致病性大肠杆菌73株,分离率为100%。采用K-B法对分离菌株进行体外药物敏感试验,根据CLSI2006标准判定结果。结果显示,猪源性大肠杆菌仅仅对阿米卡星和大部分头孢类抗菌药物的耐药率较低,对多数抗菌药物均表现为较高的耐药率,并呈现出严重的多重耐药现象,主要表现为7重耐药、8重耐药、9重耐药和10重耐药;禽源性大肠杆菌对头孢类、氨基糖苷类和硝基呋喃类抗菌药物的耐药率<30%,其他的均>75%,多重耐药主要表现为6重耐药,总体看来,其耐药性较猪源性大肠杆菌轻微。

73 strains pathogenic Escherichia coli was obtained from diarrhea piglets and died animals on breeding farm in the suburbs of Wuhan. The rate of isolation was 100 percent. Antibiotic susceptivity was tested by Kirby-bauer method. Results were assessed according to CLSI 2006. Results： The resistance rates of swine E.coli to Amikacin and most of Cephalosporins were low, but high to other antibiotics. Multiple resistances mainly were resistance to 7,8,9 and 10 antibiotics. The resistance rates of avian E.coli to Cephalosporins, Aminoglyeoside and Nitrofurans antibiotics were less than 30 percent, to other antibiotics were more than 75 percent. Multiple resistances mainly were resistance to 6 antibiotics. In general, the resistance of avian E.coli was less than that ofswine E.coli.