摘要
目的研究结缔组织生长因子在硬皮病发病过程中的可能作用和相关临床意义。方法38例SD患者活检皮肤组织(SD组)和10例正常活检皮肤组织(正常组)应用免疫组织化学LsAB法测定结缔组织生长因子,对两组结缔组织生长因子表达含量进行比较,并对硬皮病组内的病情分类、分期、有无肺间质纤维化、雷诺现象、腊肠指等的皮肤损害组织上结缔组织生长因子表达定量进行比较以及结缔组织生长因子表达定量与ANA滴度、病程有无相关性,对资料进行统计学分析。结果结缔组织生长因子阳性颗粒定位在皮肤组织细胞的胞浆内和胞膜上;实验组的结缔组织生长因子表达量显著高于对照组(T=90,P=0.000);在实验组内结缔组织生长因子表达量在病情分类、分期、有无肺间质纤维化、雷诺现象、腊肠指等没有差异(P>0.05);结缔组织生长因子表达定量与ANA滴度呈等级正相关(r=0.685,0.01<P<0.05),与病程无相关性(r=0.224,P=0.176)。结论结缔组织生长因子可能参与硬皮病的整个发病过程。
Objective To investigate the role of CTGF in the pathogenesis of scleroderma and the relationship between this kind of cytokine and clinical indexes of scleroderma. Methods The CTGF expression in lesions of 38 patients with scleroderma and 10 normal controls were detected using immunohistochemistry LsAB assay. Results The positive signal of CTGF in intracytoplasm and cell membrane in the dermal tissue. The expression of CTGF in patients with scleroderma was significantly higher than those in control group （T = 90, P = 0. 000）. The expression of CTGF was not significant （P 〉 0.05 ） between clinical indexes of scleroderma with or without the other autoimmune diseases, systemic or localized scleroderm, at the early stages or at the middle and later stages of disease, pulmonary interstitial fibrosis,allanto -finger and raynaud phenomenon respectively. The expression of CTGF had rank correlation to ANA titre （among 1：100,1：1000 and 1 ： 10000） of scleroderma （r = 0. 685,0.01 〈 P 〈 0.05 ）, and no correlation to course of scleroderma（ r = 0. 224 ,P = 0. 176）. Conclusions CTGF may palys a role in the pathogenesis of scleroderma.
出处
《医学信息（手术学分册）》
2008年第5期430-433,共4页
Medical Information Operations Sciences Fascicule
