叶下珠多酚类化合物的LC/MS分离鉴定

Separation and Determination of Polyphenols in Phyllantuus Urinaria L.by LC/MS

[目的]分析叶下珠多酚化合物的组成,为质量标准制订提供依据。[方法]叶下珠药材,以50%乙醇提取,乙酸乙酯萃取,萃取液过微孔滤膜后,采用LC/MS对各成分分离鉴定。[结果]叶下珠多酚是以芸香苷、叶下珠素和鞣花酸为主的化合物,游离的没食子酸含量很少。[结论]单以没食子酸为质量指标不合理,而以芸香苷、叶下珠素和鞣花酸总量为指标更具合理性。

[Objective]To present the basis for qualitative control by analyzing the components of polyphenols in Phyllantuus urmaria L. [Method]Raw material was extracted by 50% ethanol,then purified by acetic ethyl. The extract was injected into LC/MS after filtering with micro-membrane. [ Result] The polyphenols mainly consisted of rutin, phyllansusiin, ellagic acid, etc, but less free gallic acid. [Conclusion]The total content of these compounds should be used for qualitative control instead of gallic acid.