铁皮石斛花挥发性成分研究

Determination of chemical constituents of essential oil from flower of Dendrobium candidum Wall.ex Lind1.

目的:研究铁皮石斛花中的挥发性成分。方法:利用有机溶剂-水蒸气蒸馏法提取挥发油,用气相色谱-质谱联用(GC-MS)进行测定,色谱柱为HP-5MS5%Phenyl Methyl Siloxane(30m×0.25mm×0.25μm)弹性石英毛细管柱,结合计算机检索技术对分离的化合物进行结构鉴定,应用色谱峰面积归一化法计算各成分的相对百分含量。结果:分离出89个组分,鉴定出59个化学成分,约占76.54%。其中相对百分含量大于2.0%的分别确定为壬醛9.21%、桉叶-5,11-二烯-8,12-交酯5.55%、反-2-癸烯醛4.63%、2,3-脱氢-1,8-叶油素4.39%、正二十五烷4.03%、α-柏木醇3.69%、异土木香内酯3.65%、反式-2-庚醛3.60%、E,E-2,4-癸二烯醛2.14%、β-佛尔酮2.03%。结论:本文首次采用气相色谱-质谱联用法对铁皮石斛花挥发性成分进行提取研究。

Objective： To study the chemical constituents of volatile oil from flower of Dendrobium candidum Wall. ex Lind1. Methods： The chemical compositions of volatile oil of the plant which were obtained by steam distillation with hexane were analyzed by GC-MS equipped with a elastic quartz capillary column- HP-5MS5% Phenyl Methyl Siloxane （30 m×0.25 mm×0.25 μm）. The constituents were identified by their mass spectra. The relative percentage of the oil constituents was calculated from the GC peak areas. Results： Eighty-nine kinds of chemical constituents in Dendrobiurn candidurn Wall.ex Lindl.flower were separated; of which fifty-nine compounds representing 76.51% of the oil were characterized. Relative contents that were more than 2.0% were determined as Nonanal 9.21%, Eudesma-5,11-dien-8,12-olide 5.55%, （E）-2-Decenal 4.63%, 2,3-Dehydro- 1,8-cineole 4.39%, Pentacosane 4.03%, α -Cedrol 3.69%, Isoalantolactone 3.65%, （E）-2-Heptenal 3.60%, E,E-2,4-Decadienal 2.14%, β -Phorone 2.03%.Conclusion： This paper reports, for the first time, the composition of volatile oils of Dendrobium candidurn Wall.ex Lindl.flower by GC/MS.