摘要
本文对13株不同来源的副溶血弧菌的外膜蛋白及其抗原性进行了比较研究。13株副溶血弧菌的外膜蛋白有相似的SDS-PAGE图谱。大多数菌株有五条主要的蛋白质,其大致分子量分别为:a、69kDa;b、63kDa;c、40kDa;d、30kDa;e、28kDa。其中蛋白质b是所有菌株共有的。与吸附后的兔抗副溶血弧菌血清Western印迹法结果显示抗血清与多数副溶血弧菌菌株的外膜蛋白有一条共同的免疫反应带,其大致分子量为44kDa。
The outer membrane proteins of 13 Vibrio parahaemolyticus strains from different sources have been compared. The overall profiles were simiar. Most of the strains revealed five major proteins, designated a to e, with approximate molecular weights: a, 69kDa, b, 63kDa, c, 40kDa, d, 30kDa, e, 28kDa. Band b was detected in all strains. Immunoblotting with absorbed antisera to whole cells of Vibrio parahaemolyticus showed a strong antigenic response to protein 44kDa, which was not a major outer membrane protein, this was common to most strains examined.
出处
《中国水产科学》
CAS
CSCD
1997年第4期49-53,共5页
Journal of Fishery Sciences of China
基金
欧洲共同体"发展中国家生命科学和技术"计划的资助
关键词
水产
副溶血弧菌
外膜蛋白
抗原性
Vibrio parahaemolyticus, Outer Membrane Protein, Western Blotting