摘要
为探讨外源性雌激素对子宫细胞凋亡的影响,用免疫组化SP法,观察注射外源性17β-雌二醇后5个不同给药时程去卵巢大鼠子宫中促凋亡相关蛋白Bax和抑制凋亡相关蛋白Bcl-2的表达。结果显示,同时程相比,雌激素组(OVX+E2组)Bax表达极显著降低(P<0.01),Bcl-2表达显著增强(P<0.05);不同时程相比,OVX+E2组两种蛋白表达与假手术组(SHAM组)无显著差异,给药后期OVX组Bcl-2表达较前期增加,Bax有所减少,但无显著差异。表明雌激素缺乏初期,生理剂量的外源性雌激素可通过上调Bcl-2和下调Bax对子宫起到保护作用,后期由于机体代偿,作用则不明显。
This study was designed to know the effects of estrogen on apoptosis of uterus tissue, using the method of immuno-histochemical SP to investigate the Bcl-2 and Bax positive expressions in uterus of rats ovariectomized and treated with 17β-estrodiol in five different dosage periods. The result showed that the ovariectomized group （the group OVX） had very significantly degrade with the group of ovariectomized and treated with 17β-estrodiol （the group OVX＋E2） on expressions of Bcl-2 within the same stage （P〈0.01）, while expressions of Bax had significantly upgrade（P〈0.05） ; the expressions of Bcl-2 and Bax of the sham ovariectomized group （the group SHAM） and the group OVX＋E2 in different periods had no remarkable difference. In the later stage, the positive expressions of Bcl-2 had a slight increase than before in the group OVX, while the expressions of Bax were decreasing,and the expressions had no remarkable difference . All the results illustrated that estrogen in proper dose had certain protective effect on uterus by up-regulating the expressions of Bcl-2 and down-regulating the expressions of Bax in the initial stage of estrogen deficiency, while no significant regulative effect in the later stage of estrogen deficiency.
出处
《西北农业学报》
CAS
CSCD
北大核心
2008年第4期42-46,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
陕西省自然科学基金
西北农林科技大学科研启动基金
