不同生态类型甘蓝型油菜的SSR遗传多样性分析

Genetic Diversity Analysis on Different Types of B.napus L.by SSR Markers

利用SSR标记研究了35个甘蓝型油菜（17个春性品种（系）和15个半冬性品种（系）以及3个春性品系与半冬性品系杂交选育出来的品系）的遗传多样性及其亲缘关系。研究结果表明：①30对SSR标记共扩增出90个多态性片段,其中多态性位点占总扩增位点的92.8%,平均每对引物检测等位基因3个,片段大小介于100-1 000 bp间。②利用由非加权类平均法（UPGMA）聚类分析表明,在遗传相似系数0.698处将35份材料分为5类,第I类包括17个材料,这17个材料除22号YYC-2为半冬性外,其余的均为春性材料;第Ⅱ类包括12个材料,其中18、19、20为春性和半冬性品系杂交选育出来的品系,其他的均为半冬性材料;第Ⅲ类包括3个半冬性材料,分别为华双3号、浙油758和华双2号;第IV类包括一个春性材料Wester;第V类包括两个半冬性材料中双9号和中双10号。从上述聚类结果看出总体上春性和春性材料聚在一起,半冬性和半冬性材料聚在一起,表明春性和半冬性品种（系）的遗传差异比较大。

The genetic diversity and relationships among a collection of B. napus L. accessions , including 17 spring, 15 weak-winter and 3 hybrid lines, were investigated by simple sequence repeats （SSRs）. Two conclusions can be drawn from this study： （1）30 SSR primer pairs generated a total of 90 polymorphic bands, which took 92.8% of total amplified bands. 3 polymorphic bands in average. The molecular size was between 100-1 000 bp. （2）The result of UPGMA analysis to the data of SSR indicated that all accessions can be classified into five groups at 0. 698 similarity index level. Group Ⅰ was made up of 17 accessions, they are all spring type except YYC-2. There were 12 accessions in group Ⅱ , including three hybrid lines（ 18,19 and 20） which were created by spring and weak-winter species, others were all weak-winter types. Three weak-winter rapeseed varieties ＇Huashuang3 ＇, ＇Zheyou758＇and ＇Huashuang2＇ were in group Ⅲ. One spring varietiy Wester was in group Ⅳ. Two weak-winter rapeseed varieties ＇ Zhongshuangg＇ and ＇ Zhongshuang10＇ were in group Ⅴ. Results from cluster analysis showed that spring and weak-winter accessions were distinguished from each other and they had obvious genetic differences.