蒲公英提取物与狼毒素总黄酮主要药效作用研究

Main Medical Effect of the Extracts from Herba taraxaci and Total Flavones in Chamaejasmine

采用平板法、试管法测定两种蒲公英乙醇提取物与狼毒素总黄酮(TFC)对金黄色葡萄球菌、大肠杆菌、无乳链球菌体外抑制作用和最小抑菌浓度(MIC)以及最小杀菌浓度(MBC),利用二甲苯致小鼠耳廓肿胀试验、牛奶致热试验观察三者的抗炎、解热作用。结果表明,狼毒素总黄酮(TFC)与蒲公英40%乙醇提取物(40%HT)具有中度抑菌作用,且二者组合有显著的抑菌作用;狼毒素总黄酮40%乙醇提取物在500、250 mg/kg,90%乙醇提取物(90%HT)在500 mg/kg剂量下显著抑制二甲苯致小鼠耳廓的肿胀(P<0.01);狼毒素总黄酮不具有解热作用,两种蒲公英提取物均能显著抑制牛奶致热(P<0.01或P<0.05)。

In order to study the bacteriostasis and anti-inflammatory and antipyretic effects of the extracts from Herba taraxaci and total flavones in chamaejasmine. The bacteriostasis and the minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of three extracts on S. aureas, E. coli, S. dysagalactie were detected by plate process and test tube method. The antipyretic and anti-inflammatory action were observed by pyretic rabbits induced by intravenous injection of milk and mice with ear swelling induced by xylene. The results showed that the fraction of Herba taraxaci eluted with 40% of ethanol （40%HT） and total flavones in chamaejasmine （TFC） had midrange in hibitory effect on those bacteria, and the combination of 40% HT and TFC had significant efficacy. TFC and 40% HT at dose of 500,250 mg/kg and 90% HT at 500 mg/kg had significant inhibitory effect on ear swelling in mice（P〈0.01）. Two extracts from Herba taraxaci both had significant antipyrecit action（P〈0.01 or P〈0.05） ,but TFC didn＇t have the function.