真姬菇子实体多糖体外抗氧化特性研究

Antioxidant Properties of Polysaccharide Extracts from Fruitbodies of Hypsizigus marmoreus

以抗坏血酸vC为对照,对真姬菇子实体未脱蛋白多糖(HMPⅠ)和脱蛋白多糖(HMPⅡ)的抗氧化性进行研究。结果表明,HMPⅠ与HMPⅡ对O2-.、.OH和DPPH.均有不同程度的清除作用并有一定的还原能力。其对O2-.清除效果不明显;对.OH有较好的清除效果,且HMPⅠ对.OH清除率较HMPⅡ高;在DPPH.体系中,在浓度小于2.2 mg/mL时,HMPⅠ与HMPⅡ清除率小于vC,高于此浓度时大于vC,但两者对DPPH.清除率基本没有差别;HMPⅠ与HMPⅡ的还原能力相当,但与vC相比还原能力较弱。

The antioxidant properties of polysaccharide extracts from fruitbodies of Hypsizigus marmoreus were studied and compared with vC using superoxide radical system, hydroxyl radical system, 1,1-diphenyl-2-picrylhydrazyl radical system and reducing power. The results showed that HMP Ⅰ （non-deproteinization Hypsizigus marmoreus polysaccharides） and HMP Ⅱ （deproteinization Hypsizigus marmoreus polysaccharides） had different scavenging activity in these radical systems. Their scavenging activity to superoxide radical were not abvious, but to hydroxyl radical had a better scaw enging effectiveness and HMP Ⅰ was higher than HMP Ⅱ. With regard to the scavenging ability on 1, 1-diphenyl-2-picrylhydrazyl radicals, both HMP Ⅰ and HMPⅡ were less than vC when the concentration lower than 2.2 mg/mL and more than vC when the concentration higher than 2.2 mg/mL, but they had not significant difference. Their reducing power were similarly equal, but less than vC.