修正高斯模型在制备液相色谱收集区间优化中的应用

Optimization of the collection region in preparative high performance liquid chromatography with exponentially modified Gaussian (EMG) model

近年来制备液相色谱得到了越来越广泛的应用。制备液相色谱操作条件的优化对提高其效率及节约成本非常重要。对制备高效液相色谱而言,其样品收集区间的确定及优化是其操作条件优化的重要步骤。该文以修正高斯模型(EMG模型)为理论基础,编写了一个小软件,可有效地帮助用户确定合理的样品收集区间。此软件对制备液相色谱实际应用有积极的指导及参考意义。

Preparative high performance liquid chromatography （HPLC） are widely used recently. The optimization of its operating conditions is important for improving efficiency and saving operating cost. Now, most works such as linear scale up technology were focused on the optimization of mobile phase. The sample collection region is another important operating parameter in preparative HPLC. In this study, a software was written according to the exponentially modified Gaussian （EMG） model. With this software, the operators of preparative HPLC can easily choose a suitable sample collection region.