姜黄素对肺纤维化大鼠肺功能的影响

Effects of Curcumin on Lung Function of Pulmonary Fibrosis Rats

目的研究姜黄素对博莱霉素致大鼠肺纤维化的治疗作用。方法雄性SD大鼠144只,分为假手术组,模型组,姜黄素高、中、低剂量组,醋酸泼尼松组,每组24只。除假手术组外其余各组大鼠气管内一次性滴注盐酸博莱霉素,假手术组大鼠气管内一次性滴注等体积的生理盐水。造模后第1天开始给药,持续到处死动物的前一天。姜黄素高、中、低剂量组的剂量分别为200,100,50mg·kg-1·d-1,醋酸泼尼松组0.56mg·kg-1·d-1,假手术组,模型组分别灌服等体积的生理盐水。各组动物于7,14,28d随机处死8只,分别观察各组大鼠肺功能的变化,同时取肺组织进行HE、Mallory染色。结果姜黄素明显改善肺纤维化大鼠的肺功能。病理组织学检查亦表明,姜黄素明显改善实验性肺纤维化。结论姜黄素对博莱霉素致大鼠肺纤维化有一定的治疗作用。

Objective To observe the therapeutic effects of curcumin on pulmonary fibrosis rats induced by bleomycin. Method One hundred and forty - four male Sprague - Dawley rats were randomly divided into 6 groups （24 rats in each group）. Rats in the model control group, positive medicine group, and high -, moderate - and low -dosage curcumin groups were injected with a single dose of bleomycin through trachea, and rats in sham - model control group with the same volume of normal saline. One day after the injection, curcumin solution of different dosages （200, 100, 50 mg· kg^-1 · d^-1） was respectively given to rats in the high - , moderate - and low - dosage curcumin groups daily by gastric gavage, while the same volume of normal saline was given to those in the sham - model control group and model control group, and the same volume of prednisone（0.56 mg · kg^-1 · d^-1） was given to those in the positive medicine control group. On the 7th, 14th, and 28th day after medication, 8 rats in per treatment group were randomly killed, the changes of lung function were observed, and the lung was incised to make pathological sections which were stained with HE and Mallory. Results Curcumin improved lung function. The results of pathological examination showed that cur- cumin relieved the pathogenic changes of the lungs of pulmonary fibrosis rats induced by bleomycin. Conclusion Curcumin exerts a certain curative effect on pulmonary fibrosis rats induced by bleomycin.