摘要
目的研究Ki67反义寡核苷酸(antisense oligodexynucleotides,AS-ODN)对人前列腺癌PC-3细胞增殖的抑制作用及与紫杉醇联用的协同作用。方法Ki67反义寡核苷酸在脂质体介导下转染PC-3细胞,以RT-PCR技术检测Ki67基因表达情况,以CCK8法检测Ki67反义寡核苷酸以及联合紫杉醇对PC-3细胞增殖的影响,以金正均Q值法评价二者是否有协同作用。结果31.25nmol/LAS-ODN能显著抑制PC-3细胞的增殖(P<0.05),且抑制作用随AS-ODN浓度增加而增强;各不同浓度的AS-ODN能显著降低Ki67 mRNA的表达;31.25nmol/LAS-ODN与0.001μmol/L紫杉醇联用,Q=1.77。结论Ki67反义寡核苷酸可以通过抑制增殖蛋白Ki67的表达进而抑制PC-3细胞的增殖,且与紫杉醇(0.001μmol/L)联用有协同作用。
Objective To investigate the inhibition effect of Ki67 AS - ODN on prostate carcinoma PC - 3 cells, and its possible synergism existing in combination of Ki67 AS - ODN and paclitaxel. Methods Ki67 AS - ODN were transfected into PC - 3 cells by lipofectamine. Cell proliferation was measured by CCK8 method, and Ki67 mRNA expression was detected by RT - PCR. The synergetic effect of Ki67 AS - ODN combined with paclitaxel was evaluated by Zhengjun Jin Q method. Results AS - ODN at the concentration of 31.25 nmol /L can significantly inhibit PC - 3 cells proliferation （ P 〈 0. 05）, and the effect was dose - dependent. AS - ODN at different concentrations can decrease Ki67 mRNA expression. When 31.25 nmol/L AS - ODN were used together with 0. 001 μmol/L paclitaxel, Q = 1.77. Conclusion Ki67 AS - ODN can inhibit the proliferation of PC - 3 cells through decreasing Ki67 mRNA expression, and have a synergistic effect when combined with paclitaxel at the concentration of 0. 001μmol/L.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第4期275-277,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省医学科学技术研究基金(A2005805)
