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反义caveolin-1对实质肿瘤细胞中β3-半乳糖基转移酶7表达的调节作用 被引量：1

Down-regulation of caveolin-1 modulates the expression of β3-GalT7 in human tumor cells
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摘要 目的:研究反义caveolin-1对实质肿瘤细胞中β3-半乳糖基转移酶7(β3GalT7)表达的调节,以期阐明caveolin-1与β3GalT7的相关性。方法:运用脂质体介导转染人工合成的caveolin-1反义核苷酸(ASODN)至人类4种实质肿瘤细胞中,通过荧光显微镜、RT-PCR及蛋白质印迹检测β3GalT7表达的变化。结果:转染反义caveolin-1后的4种实质肿瘤细胞中β3GalT7均有明显的增强。结论:表明caveolin-1调节肿瘤细胞中β3GalT7的表达,且与β3GalT7的表达呈负相关。 Objective： To research the modulation of the down-regulation of caveolin-1 to β3GAlT7 in human parenchyma tumor cells. Methods： By means of the transfection to transfect the ASOND to four parenchyma tumor cells to detect the expression of β3GAlT7. Results： The expression of β3GalT7 had been raised in the four cells after transfection of ASODN. Conclusion： Caveolin-1 had a minus relationship with β3GalT7.
作者 秦芳 杨玲焰 姜智 吴祁生 徐岚 闫石 杨世蕊 吴士良
机构地区 苏州大学基础医学与生物科学学院生物化学与分子生物学教研室生物工程研究所
出处 《江苏大学学报（医学版）》 CAS 2008年第4期314-317,I0002,共5页 Journal of Jiangsu University：Medicine Edition
基金 国家自然科学基金资助项目(30670462)
关键词 β3-半乳糖基转移酶7 CAVEOLIN-1 反义核苷酸 肿瘤细胞 β3GalT7 caveolin-1 ASODN tumor cells
分类号 R730.51 [医药卫生—肿瘤] R512.620.5 [医药卫生—内科学]
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参考文献10

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同被引文献4

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江苏大学学报（医学版）
2008年 第4期

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