桂花离体胚的组织培养

Embryo Culture of Osmanthus Fragrans Lour. in Vitro

为给桂花的良种快繁奠定基础,采用不同培养基和激素对金桂胚的组织培养进行了初步研究。在胚苗培养中,进行了胚的初代萌发诱导培养、胚苗的伸长培养和生根培养,其最佳培养基配方分别为：MS＋（1.00-2.00）mg.L^-16-BA＋（0.00-0.05）mg.L-1NAA;B5＋2.00 mg.L-1GA;1/2MS＋3.00 mg.L^-1NAA。还进行了胚的愈伤组织初代诱导培养和胚苗茎段初代萌发诱导培养,其最佳培养基配方分别为：LMC＋（0.12-0.30）mg.L^-12,4-D;B5＋1.00 mg.L^-16-BA＋0.05 mg.L^-1NAA。

In order to lay a foundation for fine varieties rapid propagation, embryo culture of Osrnanthus fragrans Lour. was researched by using different media and hormones. Primary germinating induction culture, embryo seedlings elongation culture and rooting culture were carried out. The optimum culture medium for primary germinating induction culture was MS supplemented with 1. 00-2. 00 mg · L^-1 6-BA, 0. 00-0. 05 mg · L^-1 NAA; the one for embryo seedlings elongation culture was B5 supplemented with 2.00 mg · L ^-1 GA; the one for rooting culture was 1/2 MS supplemented with 3.00 mg · L ^-1 NAA. Primary callus induction and primary bud germination culture were carried out. The optimum culture medium for primary callus induction was LMC supplemented with 0.12-0.30 mg ·L^-1 2,4-D; the one for primary bud germination culture was B5 supplemented with 1. 00 mg · L^-1 6-BA and 0.05 mg · L^-1 NAA.