广西侗族人多瘤病毒感染率的调查研究

Prevalence of BK virus infection in healthy individuals of Dong minority in Guangxi

目的了解广西侗族健康人多瘤病毒(BKV)的感染情况,为防治BKV相关性肾病(BKVN)提供理论依据。方法采集广西侗族健康人外周血标本300份,提取淋巴细胞基因组DNA,用巢式PCR方法扩增BKV的保守区编码序列,统计分析不同年龄、性别组BKV-DNA检出率。结果300份广西侗族健康人BKV-DNA检出率为60.67%,不同性别组、年龄组之间差异无统计学意义(P>0.05)。结论BKV在广西侗族健康人中感染率较高,并进一步证实了外周血淋巴细胞(PBLs)是BKV在体内的潜伏细胞及传播载体,应加强对BKV条件致病性的认识。

Objective To investigate the prevalence of BK virus of healthy individuals of Dong minority in Guangxi,and to provide theory for the prevention and reduction of BKV nephrosis （BKVN）. Methods DNA in peripheral blood leukoeytes from 300 blood samples of healthy adult individuals of Dong ethnic group was extracted and BKV DNA was examined by nested polymerase chain reaction. The BKV-DNA detection rates were statistically analyzed among the groups of different ages and sex. Results In the 300 peripheral leukoeytes samples, the detection rate of BKV-DNA was 60.67%. There was no significant difference in ages or sex distribution （P 〉 0.05）. Conclusion It shows a high prevalence of BKV in peripheral blood leukoeytes of healthy individuals of Dong minority in Guangxi. This study shows that PBLs are the harbors and the transmission carriers for BKV. Awareness of the opportunistic pathogenicity of BKV should be improved so as to decrease the incidence of BKV nephrosis.