淫羊藿属主要资源种类的HPLC指纹图谱特征和种类鉴定

Studies on fingerprinting and identification of main species of Herba Epimedii

目的:淫羊藿的物种鉴定一直是中药材研究和应用中比较困难的问题,本实验旨在尝试建立一种淫羊藿药材物种鉴定方法。方法:对淫羊藿Epimedium brevicornu、箭叶淫羊藿E.sagittatum、柔毛淫羊藿E.pubescens、朝鲜淫羊藿E.koreanum、巫山淫羊藿E.wushanense、粗毛淫羊藿E.acuminatum、天平山淫羊藿E.myrianthum和黔岭淫羊藿E.leptorrhizum等8个淫羊藿药材主要来源物种,以及淫羊藿属的其他24个物种中黄酮类成分的HPLC指纹图谱进行了研究,提取可用于种类鉴定的特征。结果与结论:朝鲜淫羊藿具有的HPLC指纹特征,可以区别于其他物种,心叶淫羊藿、粗毛淫羊藿、天平山淫羊藿的大部分样品也具有可资鉴别的指纹特征,而柔毛淫羊藿和黔岭淫羊藿种内图谱模式相对稳定,但是难以与其他物种区别,箭叶淫羊藿和巫山淫羊藿则种内图谱变化十分复杂。本研究也展示出淫羊藿药材质量极其不稳定。

Objective： To establish an effective method for the identification of Herba Epimedii. Method： The HPLC finger- prints of 8 officinal species including 5 species recorded in ＇ Chinese Pharmacopoeia＇, which are Epimedium brevicornu, E. sagittatum, E. pubescens, E. koreanum and E. wushanense, and the other 3 species, E. acuminatum, E. myrianthum and E. leptorrhizum, recorded in ‘ Guizhou Quality Criteria for Traditional Chinese Medical Material and Nationality Medical Material＇ , and of other 24 species in genus Epimedium in China as well had been analyzed and some fingerprinting features for the identification had been pointed out. Result and Conclusion： The fingerprinting features of E. koreanum are rather different from others and can be used for the species identification. There are some common fingerprinting features in the most samples of E. brevicornu, E. acuminatum and E. myri-anthum, respectively, and these features are seldom found in the samples of other species. The HPLC fingerprints of E. pubescens and E. leptorrhizum appear steadily among all samples of the species respectively, but it is difficult to distinguish the spcecies from other species with the HPLC fingerprints only. The HPLC fingerprints of E. sagittatum and E. wushanense vary greatly within the species. It is also showed that the quality of Herba Epimedii varied significantly according to the HPLC features.