丛枝菌根真菌对枳利用难溶性磷酸盐及其生长的影响

Effect of Inoculated Arbuscular Mycorrhiza Fungi on Utilization of Insoluble Phosphate and the Growth in Poncirus trifoliate(L.) Raf.Seedlings

在温室沙培灭菌条件下,分别以AlPO4(Al-P)和FePO4.4H2O(Fe-P)为磷源,以枳[Poncirus trifo-liate(L.)Raf.]实生苗为试材,以摩西球囊霉(Glomus mosseae)为菌剂,研究低磷胁迫条件下接种丛枝菌根真菌对枳利用难溶性磷酸盐及生长的影响。结果表明:接种菌根菌显著增加了枳的干重、含磷量及吸磷量;未接种条件下,枳可以利用一定量的Al-P和Fe-P,但更容易利用Fe-P;接种后,枳对Al-P的利用量显著高于不接种的,随Al-P的施用量提高,菌根效应、全株吸磷量、真菌吸磷量及真菌吸磷贡献率显著增加;枳对Fe-P的利用量接种与不接种差异显著,但菌根效应、全株吸磷量、真菌吸磷量、真菌吸磷贡献率及菌根效应均低于Al-P(换算成P2O5)处理;接种处理的基质中有效磷含量显著高于未接种的。

The effect of arbuseular mycorrhizal（AM） fungi on utilization of insoluble phosphate and the growth in Poncirus trifoliate （L.） Raf. seedlings was studied in green house. The seedlings were grown in the sterilized sand amended with AI-P or Fe-P, inoculated with Glomus mosseae. Results showed that the inoculation had significant effects on AM fungi infection of the plants. The dry matter,P contents and P uptake of the plant in Poncirus trifoliate （L.） Raf. seedlings were increased. These seed lings could utilize some amount of Al-P and Fe-P without mycorrhiza fungi,but Fe-P was much easier to be used. Inoculated with mycorrhiza fungi, the utilization of Al- P was greatly increased compared with the control. Arbuscular mycorrhiza effect, P uptake by AM fungi and contribution of P uptake by AM fungi were greatly increased with the Al-P level upward. There was no great increase P uptake of Fe P compared with the control,but the P uptake of Fe-P was lower than that of Al P; Inoculated with Glomus mosseae , available P concentration in sand was significantly higher than that of controlled treatment,which was increased with the rise of P level; The spore density and the percentage of infected mycorrhizal were decreased with P level increased.