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Extraction and DNA Digestion of 5'-Phosphodiesterase from Malt Root

Extraction and DNA Digestion of 5'-Phosphodiesterase from Malt Root
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摘要 This study investigated the extraction of 5＇-phosphodiesterase from malt root and the degradation of nucleic acids by this enzyme. The extraction used grade precipitation with ammonium sulfate and enzymatic hydrolysis. Samples were assayed using the modified Bradford method and high performance liquid chromatography. The results show that 5＇-phosphodiesterase is isolated by grade precipitation with 30% and 80% saturation of ammonium sulfate and can be utilized to degrade deoxyribonucleic acid. The hydrolysate has four kinds of deoxynucleotides： 5＇-dCMP, 5＇-dTMP, 5＇-dAMP, and 5＇-dGMP. The optimum reaction temperature is 70℃, and the optimum pH is 5.5-6.0 for the reaction. The percentage of deoxynucleotides indicated by the China Pharmacopoeia （2000 edition） in the product is over 70%. The extraction of 5＇-phosphodiesterase from malt root is shown to be possible and economical. Products from the enzYmatic hydrolysate of DNA meet the pharmacopoeia. This study investigated the extraction of 5＇-phosphodiesterase from malt root and the degradation of nucleic acids by this enzyme. The extraction used grade precipitation with ammonium sulfate and enzymatic hydrolysis. Samples were assayed using the modified Bradford method and high performance liquid chromatography. The results show that 5＇-phosphodiesterase is isolated by grade precipitation with 30% and 80% saturation of ammonium sulfate and can be utilized to degrade deoxyribonucleic acid. The hydrolysate has four kinds of deoxynucleotides： 5＇-dCMP, 5＇-dTMP, 5＇-dAMP, and 5＇-dGMP. The optimum reaction temperature is 70℃, and the optimum pH is 5.5-6.0 for the reaction. The percentage of deoxynucleotides indicated by the China Pharmacopoeia （2000 edition） in the product is over 70%. The extraction of 5＇-phosphodiesterase from malt root is shown to be possible and economical. Products from the enzYmatic hydrolysate of DNA meet the pharmacopoeia.
作者 邹和昌 蔡光圻 蔡文 李海龙 顾益 朴龙斗 孟凡国
机构地区 Department of Biological Sciences and Biotechnology Zhejiang Jiaxing Pharmacology Corporation Yangtze Delta Region Institute of Tsinghua University
出处 《Tsinghua Science and Technology》 SCIE EI CAS 2008年第4期480-484,共5页 清华大学学报（自然科学版（英文版）
基金 the Key Technologies Research & Development Pro-gram of Zhejiang Province (No. 2005C22053)
关键词 5＇-phosphodiesterase malt root deoxyribonucleic acid 5＇-phosphodiesterase malt root deoxyribonucleic acid
分类号 Q943.2 [生物学—植物学]
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参考文献12

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二级参考文献11

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Tsinghua Science and Technology
2008年 第4期

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