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胃癌细胞在右旋甲硫氨酸中氨基酸代谢的研究

The amino acids metabolism of gastric cancer cells in D-methionine medium
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摘要 目的:研究胃癌细胞在右旋甲硫氨酸(D-Met)环境中氨基酸的代谢。方法:将6株胃癌细胞分别接种于D-Met、Met-和左旋甲硫氨酸(L-Met)培养液中,培养5 d后,用高效液相色谱法检测培养液中20种氨基酸的浓度。结果:Met-组6株胃癌细胞的Met含量显著低于L-Met组和D-Met组(P<0.05);D-Met组和Met-组SUN-1、KATO-Ⅲ和MKN45细胞的同型半胱氨酸(Hcy)含量显著高于L-Met组(P<0.05);除SUN-1和MKN45外,D-Met组和Met-组各株细胞Gln含量显著高于L-Met组(P<0.05);D-Met组和Met-组KATO-Ⅲ、NCI-N87和AGS的丙氨酸(Ala)含量显著低于L-Met组(P<0.05);D-Met组和Met-组SUN-16、KATO-Ⅲ和AGS的半胱氨酸(Cys)含量显著低于L-Met组(P<0.05)。结论:D-Met环境可影响胃癌细胞代谢,其效果与Met-环境相似。 Objective ： To investigate the amino acids metabolism of gastric cancer cells in D-methionine （D-Met） medium. Methods ： Six gastric cancer cell lines were respectively cultured with L-methionine （ L-Met）, D-Met, or without methionine （Meta^-） medium. Amino acids profile of every culture medium was measured by HPLC after 5 days. Results ： Methionine concentration of six cell lines in Met-medium was significantly lower than that in L-Met or D-Met medium （P〈0.05）. Homocysteine concentration of SUN-1, KATO-Ⅲ, and MKN45 cell lines in D-Met or Met medium was significantly higher than that in L-Met medium（P 〈0.05）. Glutamine concentration of all cell lines but SUN-1 and MKN45 in D-Met or Met-group was significantly higher than that in L-Met medium（P〈0.05）. Alanine concentration of KATO-Ⅲ ,NCI-N87 and AGS cell lines in D-Met or Met^- group was significantly lower than that in L-Met medium（P〈0.05）. Cysteine concentration of SUN-16, KATO-Ⅲ, and AGS cell lines in D-Met or Met medium was significantly lower than that in L-Met medium（P〈0.05）. Conclusion ： D-methio-nine environment could affect amino acids metabolism of gastric cancer cells, and its effect was similar to Met-environment.
作者 刘冀红 曹伟新 商俊 刘炳亚 朱正纲 林言箴
机构地区 上海交通大学附属瑞金医院临床营养科 上海交通大学附属瑞金医院上海消化外科研究所 上海交通大学附属瑞金医院普通外科
出处 《肠外与肠内营养》 CAS 2008年第4期197-200,共4页 Parenteral & Enteral Nutrition
关键词 氨基酸 胃癌 右旋甲硫氨酸 Amino acid Gastric cancer D-methionine
分类号 R735.2 [医药卫生—肿瘤]
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参考文献8

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二级参考文献15

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肠外与肠内营养
2008年 第4期

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