鱼油对腹腔感染并发腹腔高压大鼠肺功能的保护作用

Enteral fish oil immunonutrition prevents pulmonary dysfunction during intra-abdominal sepsis and hypertension in rats

目的:探讨鱼油对腹腔感染并发腹腔高压时肺功能的保护作用。方法:选用健康SD大鼠25只,随机分为等渗盐水组(n=9)、0.3%鱼油组(n=8)和0.6%鱼油组(n=8)。腹腔感染并发腹腔高压制模手术前2周开始,分别用等渗盐水0.3%鱼油和0.6%鱼油灌胃,术后24 h处死动物,观察各组大鼠肺组织病理改变、MPO阳性细胞浸润和TNF-α和IL-1表达的差异。结果:与等渗盐水组比,鱼油灌胃能显著降低腹腔感染并发腹腔高压大鼠肺组织中TNF-α和IL-1的表达,并抑制炎症细胞浸润,减轻肺组织结构的损伤。结论:鱼油有减轻炎症反应和保护器官的作用。

To investigate whether an enteral fish oil immunonutrition during intra-abdominal sepsis and hypertension dose improve pulmonary function.Methods：We divided 25 rats into three groups. The rats were supplemented by gavage with：（Ⅰ） normal saline as control group （9） ; （Ⅱ） fish oil（0.3%, 8） and （Ⅲ）fish oil（0.6%, 8）. The cytokine levels were analyzed. The lungs were harvested, stained with hematoxylin-eosin, and analyzed. Results：Intra-abdominal sepsis and hypertension induced thickening of alveolar wails and inflammatory reaction. Fish oil decreased expression of pro-in-flammatory cytokines （TNF-α and IL-1β）, inhibitted infiltration of MPO positive ceils, and protected pulmonary structure. Conclusion：The lung may be protected by an enteral fish oil nutrition through at-tenuating inflammatory reaction.