间皮素与CA125在卵巢癌中的共定位表达及意义

Expressions and significance of mesothelin and CA125 in human epithelial ovarian cancer

目的通过对上皮性卵巢癌中间皮素和CA125的表达、定位和细胞黏附的研究,探讨间皮素的功能。方法采用间接免疫荧光双标记染色法、Western blot检测间皮素和CA125的表达及定位,通过人工基底膜成分的黏附实验和EDTA介导的细胞分离实验检测细胞黏附功能。结果间皮素和CA125主要分布在细胞膜,间皮素蛋白在3种细胞系表达由强到弱依次为OVCAR-3、SKOV-3、3AO,3AO和SKOV-3对塑料基质和人工基底膜Matrigel的黏附能力明显低于OVCAR-3(P<0.01)。结论在3种卵巢癌细胞系和卵巢癌组织中间皮素和CA125均有不同程度的表达,存在共定位关系,间皮素的表达与细胞黏附功能相关联。

Objective To investigate the function of mesothelin by analyzing the location, the expressions and the adhesive ability of mesothelin and CA125 in human epithelial ovarian cancer. Methods Mesothelin and CA125 protein expressions and locations in human epithelial ovarian cancer tissues and cell lines were de- termined by indirect imnmnofluorescence double maker and Western blot. Cell adhesive ability was detected by EDTA-induced cell detachment. Results Mesothelin and CA125 were specifically located in cytomembrane of human epithelial ovarian cancer cells. Expressions of mesothelin in the SKOV-3 and 3AO cells were weaker than those in the OVCAR-3. SKOV-3 and 3AO cells adhered less effectively to plastic substance and matrigel than OVCAR-3 （P 〈0. 01 ）. Conclusion Different expressions and co-location of mesothelin and CA125 were found in in the 3 kinds of human epithelial ovarian cancer tissues and cell lines. The expression level of mesothe- lin may be related to cell adhesive ability.