低氧复合氰化钠中毒对兔动脉血超氧化物歧化酶、还原型谷胱甘肽和丙二醛的影响

Effects of hypoxia and sodium cyanide on superoxide dismutase,reduced glutathione and malondialdehyde in arterial blood of rabbits

目的探讨高原低氧复合氰化钠中毒对兔动脉血氧化应激的影响。方法以低压氧舱模拟4000m高原急性缺氧环境。20只家兔按随机数字表法分为4组,高原高、低剂量组,平原高、低剂量组,每组5只。高原缺氧组动物置于低压氧舱内预处理72h后进行实验,平原组动物于普通实验环境下进行。以戊巴比妥钠(40mg/kg,腹腔注射)麻醉动物后进行股动脉插管,注射氰化钠(1.5、2mg/kg,腹腔注射)。分别于中毒前10min和中毒后5、10、15、20、30、60、120、180min经插管采血并分离血浆,以生化方法测定超氧化物歧化酶(superoxide dismutase,SOD)活性、还原型谷胱甘肽(reducedglutathione,GSH)和丙二醛(malondialdehyde,MDA)含量。结果平原动物NaCN中毒60min后血浆MDA含量增加(P<0.01),SOD活力和GSH含量下降(P<0.05,P<0.01)。高原缺氧72h后也使MDA含量增加(P<0.01),SOD活力和GSH含量下降(P<0.05,P<0.01),高原缺氧动物注射氰化钠30min后上述指标变化较平原动物更为显著(P<0.01)。结论单纯缺氧和单纯氰化钠中毒均明显改变氧化应激指标,二者可能具有联合效应。

Objective To investigate the effects of hypoxia and sodium cyanide （NaCN） on oxidative stress in rabbit arterial blood. Methods An artificial hypobaric hypoxia chamber was used to simulate 4 000-meter high altitude. Twenty rabbits were randomly divided into 4 groups： hypoxia group with high- or low-dose, non-hypoxia with high- or low-dose. The animals in the non-hypoxia groups were operated under normal circumstances while those in hypoxia groups were subjected to chamber in low pressure for 72 h before receiving the hypoxia experiments. Femoral arterial cannulation was performed on all animals under anesthetization with pentobarbital sodium （30 mg/kg, iv） and NaCN（ip） at the doses of 1.5 mg/kg and 2 mg/kg. Blood samples were collected at 10 min before intoxication, and 5, 10, 15, 20, 30, 60, 120 and 180 min after intoxication and blood seperation was conducted. The activity of superoxide dismutase （ SOD）, contents of reduced glutathione （GSH） and malondialdehyde （MDA） were determined. Results The MDA content was significandy increased （P 〈0. 01 ）, and the SOD activity and GSH level were obviously decreased by NaCN injections in the non-hypoxia animals （P 〈0. 05, P 〈 0. 01 ）. Similar changes were also detected under the hypoxia condition （P 〈0. 01 ） 72 h later yet, and the changes of the aboving indices were more distinct in hypoxia groups than in non-hypoxia groups 30 min after intoxication. Conclusion Either hypoxia or NaCN can produce significant changes on the oxidative stress system and theg may heve a synergetic effect.